Journal: Allergologia et immunopathologia

Article Title: PTPRO activates TLR4/NF-κB signaling to intensify lipopolysaccharide-induced pneumonia cell injury.

doi: 10.15586/aei.v50i3.589

Figure Lengend Snippet: Figure 2 Cytotoxicity of PTPRO in HFL1. (A) Transfection with pcDNA-PTPRO or shPTPRO increased or decreased PTPRO expression, respectively. (B) Overexpression of PTPRO reduced cell viability of HFL1, while silencing of PTPRO did not affect cell viability. (C) Overexpression of PTPRO promoted cell apoptosis of HFL1, while silencing of PTPRO did not affect cell apoptosis. *p < 0.05, **p < 0.01.

Article Snippet: Human lung fibroblasts (HFL1) (ATCC, Manassas, VA, USA) were cultured in DMEM medium containing 10% fetal bovine Table 1 Primers.

Techniques: Transfection, Expressing, Over Expression

Journal: Allergologia et immunopathologia

Article Title: PTPRO activates TLR4/NF-κB signaling to intensify lipopolysaccharide-induced pneumonia cell injury.

doi: 10.15586/aei.v50i3.589

Figure Lengend Snippet: Figure 1 Lipopolysaccharide induced the elevation of PTPRO in HFL1. (A) Incubation with lipopolysaccharide reduced cell viability of HFL1 in a dose-dependent way. (B) Incubation with lipopolysaccharide enhanced protein expression of PTPRO in HFL1 in a dose-dependent way. *p < 0.05, ***p < 0.001.

Article Snippet: Human lung fibroblasts (HFL1) (ATCC, Manassas, VA, USA) were cultured in DMEM medium containing 10% fetal bovine Table 1 Primers.

Techniques: Incubation, Expressing

Journal: Allergologia et immunopathologia

Article Title: PTPRO activates TLR4/NF-κB signaling to intensify lipopolysaccharide-induced pneumonia cell injury.

doi: 10.15586/aei.v50i3.589

Figure Lengend Snippet: Figure 3 PTPRO contributed to lipopolysaccharide-induced apoptosis in HFL1. (A) Transfection with pcDNA-PTPRO reduced the cell viability of lipopolysaccharide-induced HFL1, while transfection with shPTPRO enhanced cell viability. (B) Overexpression of PTPRO increased cell apoptosis of lipopolysaccharide-induced HFL1, while transfection with shPTPRO inhibited cell apoptosis. (C) The apoptotic ratio of lipopolysaccharide-induced HFL1 that was transfected with pcDNA-PTPRO or shPTPRO. #p < 0.05; ##,^^p < 0.01; ***,###p < 0.001.

Article Snippet: Human lung fibroblasts (HFL1) (ATCC, Manassas, VA, USA) were cultured in DMEM medium containing 10% fetal bovine Table 1 Primers.

Techniques: Transfection, Over Expression

Journal: Allergologia et immunopathologia

Article Title: PTPRO activates TLR4/NF-κB signaling to intensify lipopolysaccharide-induced pneumonia cell injury.

doi: 10.15586/aei.v50i3.589

Figure Lengend Snippet: Figure 4 PTPRO contributed to lipopolysaccharide-induced inflammation in HFL1. (A) mRNA expressions of TNF-α, IL-1β, and IL-18 in lipopolysaccharide-induced HFL1 were enhanced by PTPRO overexpression, while they were reduced by PTPRO silence. (B) Protein expressions of TNF-α, IL-1β, and IL-18 in lipopolysaccharide-induced HFL1 were enhanced by PTPRO overexpression, while they were reduced by PTPRO silence.

Article Snippet: Human lung fibroblasts (HFL1) (ATCC, Manassas, VA, USA) were cultured in DMEM medium containing 10% fetal bovine Table 1 Primers.

Techniques: Over Expression

Journal: Allergologia et immunopathologia

Article Title: PTPRO activates TLR4/NF-κB signaling to intensify lipopolysaccharide-induced pneumonia cell injury.

doi: 10.15586/aei.v50i3.589

Figure Lengend Snippet: Figure 5 PTPRO contributed to lipopolysaccharide-induced activation of TLR4/NF-κB in HFL1. (A) Protein expressions of TLR4, p-p65, and p-IκBα in lipopolysaccharide-induced HFL1 were increased by PTPRO overexpression, while they were decreased by PTPRO silence. #,^p < 0.05; ^^p < 0.01, ***,###p < 0.001.

Article Snippet: Human lung fibroblasts (HFL1) (ATCC, Manassas, VA, USA) were cultured in DMEM medium containing 10% fetal bovine Table 1 Primers.

Techniques: Activation Assay, Over Expression

Journal: Scientific Reports

Article Title: Plant miRNA osa-miR172d-5p suppressed lung fibrosis by targeting Tab1

doi: 10.1038/s41598-023-29188-6

Figure Lengend Snippet: Osa-miR172d-5p identified as a plant miR candidate with an anti-fibrotic effect. ( A ) Scheme of plant miR selection. ( B ) In silico analysis of the interaction between osa-miR172d-5p and TAB1. ( C ) Human lung fibroblast HFL1 cells were transfected with the osa-miR172d-5p for 48 h, and TAB1 expression was evaluated via western blot analysis ( n = 4). ( D ) HFL1 cells were transfected with the indicated concentration of osa-miR172d-5p for 48 h, and cDNA was evaluated via qRT-PCR ( n = 4). Data are shown as mean ± SEM. * P < 0.05. *** P < 0.001 versus control group.

Article Snippet: Human lung fibroblast HFL1 cells (JCRB, Osaka, Japan) were cultured in 10% fetal bovine serum (FBS) (Sigma-Aldrich), Dulbecco's Modified Eagle Medium (DMEM) (044-29765; Fujifilm Tokyo, Japan) supplemented with penicillin G (876111)–streptomycin (876161; Meiji Pharmaceutical Co., Tokyo, Japan) in 5% CO 2 and 100% humidity at 37 °C.

Techniques: Selection, In Silico, Transfection, Expressing, Western Blot, Concentration Assay, Quantitative RT-PCR, Control

Journal: Scientific Reports

Article Title: Plant miRNA osa-miR172d-5p suppressed lung fibrosis by targeting Tab1

doi: 10.1038/s41598-023-29188-6

Figure Lengend Snippet: TAB1 knockdown suppressed TGFβ-induced fibrotic gene expression. ( A ) Human lung fibroblast HFL1 cells were transfected with TAB1-siRNA (10 nM, 48 h) and TAB1 expression levels were determined by western blot analysis. ( B – D ) Human lung fibroblast HFL1 cells were transfected with TAB1-siRNA (10 nM, 48 h) and treated with TGFβ (5 ng/mL for B , D 48 h; C 24 h). mRNA expression levels were assessed via RT-qPCR. ( B ) ASMA (αSMA) (n = 4), ( C ) COL1A1 (n = 4), and ( D ) FN (fibronectin; n = 4). Data are shown as mean ± SEM. * P < 0.05. ** P < 0.01. *** P < 0.001 versus control group.

Article Snippet: Human lung fibroblast HFL1 cells (JCRB, Osaka, Japan) were cultured in 10% fetal bovine serum (FBS) (Sigma-Aldrich), Dulbecco's Modified Eagle Medium (DMEM) (044-29765; Fujifilm Tokyo, Japan) supplemented with penicillin G (876111)–streptomycin (876161; Meiji Pharmaceutical Co., Tokyo, Japan) in 5% CO 2 and 100% humidity at 37 °C.

Techniques: Knockdown, Gene Expression, Transfection, Expressing, Western Blot, Quantitative RT-PCR, Control